Tomato spotted wilt virus (TSWV) is a viral disease agent that limits pepper production and causes economic losses in the world. The gene controlling resistance to TSWV (Tsw) has been detected in many Capsicum chinense pepper genotypes ('PI 152225', 'PI 159236', 'CNPH 275''C00943', and '7204') and mapped on chromosome 10 it has been mapped to cultivars (Capsicum annum). Although the homolog of Sw-5 could not be mapped, phenotypically similar genes are present in tomatoes, with many Sw5 homologs found in similar regions in tomatoes and pepper. The relationship between Tsw and Sw5 has been studied through genetic studies of TSWV. The most effective and environmentally friendly way to combat this viral pathogen is to use resistant varieties. Our study investigated the resistance levels of 40 genotypes at S3 stage against tomato spotted wilt virus (TSWV) investigated. SCAR marker was used in molecular studies. Genotypes were determined as 5 homozygous resistant (RR), 15 sensitive (rr), and 20 heterozygous resistants (Rr).