Determınatıon Of The Suıtable Nutrıent Medıum For Use Of Immature Embryo Culture In Safflower (Carthamus tinctorius) Different Genotypes

Ayşe Nuran ÇİL

doi:https://doi.org/10.54498/ETBD.2024.39

Ayşe Nuran ÇİL, Abdullah ÇİL, Hacer Burun, Rüştü Hatipoğlu, Zeynep Üçdağ

Year : 2025
Vol : 5
Issue : 1
Page : 24-32

DOI : https://doi.org/10.54498/ETBD.2024.39

Despite its adequacy in many agricultural products, Türkiye has a large deficit in vegetable oil production. For this reason, it is necessary to focus on the studies conducted on new oil crops that will contribute to vegetable oil production and be effective in eliminating our oil deficit. Safflower is one of the plants that make up this group. In this study, it was aimed to determine the most suitable nutrient medium in order to speed up the obtaining homozigot lines in the breeding programs of safflower through the immature embryo culture technique in different Safflower genotypes. The study was carried out at the Speed Breeding Center of the Eastern Mediterranean Agricultural Research Institute. In the study, five safflower genotypes (Line-1, Line-2, Line-3, Line-4 and Dinçer) and three media (MS, NN and B5) were tested. Immature embryos isolated 14 days after pollination from the seeds of plants grown under open field conditions were tested. Plant weight, plant root length, plant height and number of leaves per plant were investigated in the regenerated plants from the cultured embryos. The research findings showed that the average values of plant weight, plant root length, plant height, number of leaves per plantlet and regeneration rate varied as 0.15-0.28 g, 2.22-2.84 cm, 1.59-1.80 pieces, 1.35-1.59 cm, respectively, depending on the media. The averaged values of the mentioned characteristics varied as 0.14-0.27 g, 1.41-1.61 cm, 2.09-3.12, 1.53-1.82 pieces and 0.8-33.8% respectively, depending on the genotypes. Based on the research results, it was concluded that the immature embryo culture technique could be used to accelerate the obtainment of homozygous lines in safflower breeding, that the genotype and nutrient medium significantly affect the application efficiency of the technique, and that the nutrient medium to be used is dependent on the genotype.

Cite this Article As : Çil, A. N., & Çil, A. & Burun, H., & Üçdağ, Z. & Hatipoğlu, R. (2025). Determination of the suitable nutrient medium for the immature embryo culture in different genotypes of safflower (Carthamus tinctorius L.). Ereğli Tarım Bilimleri Dergisi, 5(1), 24-32. https://doi.org/10.54498/ETBD.2025.39

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Determınatıon Of The Suıtable Nutrıent Medıum For Use Of Immature Embryo Culture In Safflower (Carthamus tinctorius) Different Genotypes, Research Article,
2025, Vol. 5 (1)

Received : 14.04.2025, Accepted : 16.06.2025 , Published Online : 30.06.2025