This study evaluated the effects of two different pumpkin genotypes (C-26 and C-27) on root growth parameters and some nutrition element concentrations under drought stress conditions. The research was carried out in five replications. Both genotypes are negatively affected by drought stress. However, it was determined that the C-26 genotype showed a more significant decrease in root growth rates than C-27 (48%). However, while was an increase in root fresh and dry weights in the C-26 genotype, there were decreases in the C-27 genotype compared to the control. A proportionally more significant decrease in Ca, Mg, and Na contents in the C-26 genotype was observed with drought stress. In the amount of K, the C-27 genotype showed a significant decrease compared to C-26. Having information about the contents of plant nutrients in drought conditions in plants with economic potential such as zucchini can help control plants' growth and development under these conditions.